SPHK2 antibody (AA 215-269) (DyLight 550)

Catalog No. ABIN7354616

This Rabbit Polyclonal antibody specifically detects SPHK2 in FACS. It exhibits reactivity toward Human.

Quick Overview for SPHK2 antibody (AA 215-269) (DyLight 550) (ABIN7354616)

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK2 antibody is conjugated to DyLight 550

Application: Flow Cytometry (FACS)

Product Details anti-SPHK2 Antibody (DyLight 550)

Binding Specificity: AA 215-269

Purpose: Anti-Human SPHK2 DyLight® 550 conjugated Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human SPHK2 DyLight® 550 conjugated Antibody -Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human SPHK2 recombinant protein (Position: F215-M269).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Hait NC, Sarkar S, Le Stunff H, Mikami A, Maceyka M, Milstien S, Spiegel S (August 2005). "Role of sphingosine kinase 2 in cell migration toward epidermal growth factor". The Journal of Biological Chemistry. 280 (33): 29462-9. 2. Liu H, Sugiura M, Nava VE, Edsall LC, Kono K, Poulton S, Milstien S, Kohama T, Spiegel S (June 2000). "Molecular cloning and functional characterization of a novel mammalian sphingosine kinase type 2 isoform". The Journal of Biological Chemistry. 275 (26): 19513-20.

Comment:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for SPHK2

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Alternative Name: SPHK2

Background:

Synonyms: Sphingosine kinase 2, SK 2, SPK 2

Background: Sphingosine kinase 2 is a protein that in humans is encoded by the SPHK2 gene. This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Molecular Weight: 39 kDa

Gene ID: 56848

Pathways: VEGF Signaling