SP1 antibody (AA 384-603)

Catalog No. ABIN6719376

This anti-SP1 antibody is a Rabbit Polyclonal antibody detecting SP1 in WB, ELISA, IHC, IF, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for SP1 antibody (AA 384-603) (ABIN6719376)

Target: SP1 (Sp1 Transcription Factor (SP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-SP1 Antibody

Binding Specificity: AA 384-603

Purpose: Anti-SP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SP1 Antibody Picoband® (ABIN6719376). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human SP1 recombinant protein (Position: Q384-A603).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Immunofluorescence, 5 μg/mL
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Gaynor, R. B., Shieh, B.-H., Klisak, I., Sparkes, R. S., Lusis, A. J.Localization of the transcription factor SP1 gene to human chromosome 12q12-q13.2.Cytogenet. Cell Genet. 64: 210-212, 1993. 2. Matera, A. G., Ward, D. C.Localization of the human Sp1 transcription factor gene to 12q13 by fluorescence in situ hybridization.Genomics 17: 793-794, 1993. 3. Wimmer, E. A., Jackle, H., Pfeifle, C., Cohen, S. M.A Drosophila homologue of human Sp1 is a head-specific segmentation gene.Nature 366: 690-694, 1993.Wimmer, E. A., Jackle, H., Pfeifle, C., Cohen, S. M.A Drosophila homologue of human Sp1 is a head-specific segmentation gene.Nature 366: 690-694, 1993.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SP1

Target: SP1 (Sp1 Transcription Factor (SP1))

Alternative Name: SP1

Background:

Synonyms: Transcription factor Sp1, SP1, TSFP1

Tissue Specificity: Up-regulated in adenocarcinomas of the stomach (at protein level). Isoform 3 is ubiquitously expressed at low levels.

Background: Transcription factor Sp1, also known as specificity protein 1* is a protein that in humans is encoded by the SP1 gene. The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 90 kDa

UniProt: P08047

Pathways: Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction