SRI antibody

Catalog No. ABIN6719388

This anti-SRI antibody is a Rabbit Polyclonal antibody detecting SRI in WB, IHC, IF, ICC, FACS and IHC (fro). Suitable for Human, Mouse and Rat.

Quick Overview for SRI antibody (ABIN6719388)

Target: SRI (Sorcin (SRI))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRI antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SRI Antibody

Purpose: Anti-SRI Antibody Picoband®

Sequence: TVDPQELQKA LTTMGFRLSP QAVNSIAKRY

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SRI Antibody Picoband® (ABIN6719388). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SRI, which shares 93.3% amino acid (aa) sequence identity with both mouse and rat SRI.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1. "Entrez Gene: SRI sorcin". 2. Meyers, M B, Pickel V M, Sheu S S, Sharma V K, Scotto K W, Fishman G I (Nov 1995). "Association of sorcin with the cardiac ryanodine receptor". J. Biol. Chem. (UNITED STATES) 270 (44): 26411-8. 3. Van der Bliek AM, Baas F, Van der Velde-Koerts T, Biedler JL, Meyers MB, Ozols RF, Hamilton TC, Joenje H, Borst P (Nov 1988). "Genes amplified and overexpressed in human multidrug-resistant cell lines". Cancer Res48 (21): 5927-32. 

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SRI

Target: SRI (Sorcin (SRI))

Alternative Name: SRI

Background:

Synonyms: Sorcin, 22 kDa protein, CP-22, CP22, V19, SRI

Tissue Specificity: Detected in cardiac myocytes.

Background: Sorcin?is a?protein?that in humans is encoded by the?SRI?gene. It is mapped to 7q21.1. This gene encodes a calcium-binding protein with multiple E-F hand domains that relocates from the cytoplasm to the sarcoplasmic reticulum in response to elevated calcium levels. In addition to regulating intracellular calcium homeostasis it also modulates excitation-contraction coupling in the heart. Alternative splicing results in multiple transcript variants encoding distinct proteins. Multiple pseudogenes exist for this gene.?

Molecular Weight: 22 kDa

Gene ID: 6717

UniProt: P30626

Pathways: Transition Metal Ion Homeostasis, Maintenance of Protein Location, Negative Regulation of Transporter Activity