PARN antibody (AA 1-301)

Catalog No. ABIN6719422

This Mouse Monoclonal antibody specifically detects PARN in WB, IHC, IF, ICC and FACS. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for PARN antibody (AA 1-301) (ABIN6719422)

Target: PARN (Poly A Specific Ribonuclease (PARN))

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This PARN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 11D8

Product Details anti-PARN Antibody

Binding Specificity: AA 1-301

Purpose: Anti-PARN Antibody Picoband® (monoclonal, 11D8)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PARN Antibody Picoband® (monoclonal, 11D8) (ABIN6719422). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human PARN recombinant protein (Position: M1-Y301).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1."Entrez Gene: PARN poly(A)-specific ribonuclease (deadenylation nuclease)". Buiting K, Korner C, Ulrich B, Wahle E, Horsthemke B (May 2000). "The human gene for the poly(A)-specific ribonuclease (PARN) maps to 16p13 and has a truncated copy in the Prader-Willi/Angelman syndrome region on 15q11→q13". Cytogenet Cell Genet. 87 (1-2): 125-31.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PARN

Target: PARN (Poly A Specific Ribonuclease (PARN))

Alternative Name: PARN

Background:

Synonyms: Poly (A)-specific ribonuclease PARN, Deadenylating nuclease, Deadenylation nuclease, Polyadenylate-specific ribonuclease, PARN, DAN

Tissue Specificity: Ubiquitous.

Background: Poly (A)-specific ribonuclease (PARN), also known as polyadenylate-specific ribonuclease or deadenylating nuclease (DAN), is an enzyme that in humans is encoded by the PARN gene. The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly (A) as the substrate, hence, efficiently degrades poly (A) tails of mRNAs. Exonucleolytic degradation of the poly (A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons.

Molecular Weight: 78 kDa

Gene ID: 5073

UniProt: O95453