Pyrophosphatase (Inorganic) 1 (PPA1) (AA 7-236) antibody

Catalog No. ABIN6719527

This Rabbit Polyclonal antibody specifically detects in WB, ELISA and IHC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Pyrophosphatase (Inorganic) 1 (PPA1) (AA 7-236) antibody (ABIN6719527)

Target: Pyrophosphatase (Inorganic) 1 (PPA1)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details

Binding Specificity: AA 7-236

Purpose: Anti-Pyrophosphatase 1/PPA1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Pyrophosphatase 1/PPA1 Antibody Picoband® (ABIN6719527). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Pyrophosphatase 1/PPA1 recombinant protein (Position: E7-N236).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Curbo, S., Lagier-Tourenne, C., Carrozzo, R., Palenzuela, L., Lucioli, S., Hirano, M., Santorelli, F., Arenas, J., Karlsson, A., Johansson, M. Human mitochondrial pyrophosphatase: cDNA cloning and analysis of the gene in patients with mtDNA depletion syndromes. Genomics 87: 410-416, 2006. 2. Fairchild, T. A., Patejunas, G. Cloning and expression profile of human inorganic pyrophosphatase. Biochim. Biophys. Acta 1447: 133-136, 1999.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details

Target: Pyrophosphatase (Inorganic) 1 (PPA1)

Alternative Name: PPA1

Background:

Synonyms: Inorganic pyrophosphatase, Pyrophosphate phospho-hydrolase, Ppase, PPA1, IOPPP, PP

Tissue Specificity: Expressed ubiquitously.

Background: PPA1 is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. By radiation hybrid analysis, the PPA1 gene is mapped to chromosome 10q21.3.

Molecular Weight: 33 kDa

Gene ID: 5464

UniProt: Q15181