CA2 antibody (AA 2-260)

Catalog No. ABIN6719533

This Mouse Monoclonal antibody specifically detects CA2 in WB, IHC, ICC, IF and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for CA2 antibody (AA 2-260) (ABIN6719533)

Target: CA2 (Carbonic Anhydrase II (CA2))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 10E11

Product Details anti-CA2 Antibody

Binding Specificity: AA 2-260

Purpose: Anti-CA2 Antibody Picoband® (monoclonal, 10E11)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CA2 Antibody Picoband® (monoclonal, 10E11) (ABIN6719533). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CA2 recombinant protein (Position: S2-K260). Human CA2 shares 81.1% and 80.7% amino acid (aa) sequence identity with mouse and rat CA2, respectively.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1.Aramaki, S., Yoshida, I., Yoshino, M., Kondo, M., Sato, Y., Noda, K., Jo, R., Okue, A., Sai, N., Yamashita, F. Carbonic anhydrase II deficiency in three unrelated Japanese patients. J. Inherit. Metab. Dis. 16: 982-990, 1993. 2.Laitala, T., Vaananen, H. K. Inhibition of bone resorption in vitro by antisense RNA and DNA molecules targeted against carbonic anhydrase II or two subunits of vacuolar H(+)-ATPase. J. Clin. Invest. 93: 2311-2318, 1994. 3.Venta, P. J., Welty, R. J., Johnson, T. M., Sly, W. S., Tashian, R. E. Carbonic anhydrase II deficiency syndrome in a Belgian family is caused by a point mutation at an invariant histidine residue (107his-to-tyr): complete structure of the normal human CA II gene. Am. J. Hum. Genet. 49: 1082-1090, 1991.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Alternative Name: CA2

Background:

Synonyms: Carbonic anhydrase 2, Carbonate dehydratase II, Carbonic anhydrase C, CAC, Carbonic anhydrase II, CA-II, CA2

Tissue Specificity: Ubiquituous. Expressed in platelets.

Background: CA2 is a cytosolic enzyme with the highest activity among all known CAs. The carbonic anhydrases (ACs) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs relatively slowly in the absence of a catalyst. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. This gene is mapped to 8q22.

Molecular Weight: 28 kDa

Gene ID: 760

UniProt: P00918