ADO antibody (AA 49-261) (DyLight 550)

Catalog No. ABIN7354747

The Rabbit Polyclonal anti-ADO antibody has been validated for FACS. It is suitable to detect ADO in samples from Human.

Quick Overview for ADO antibody (AA 49-261) (DyLight 550) (ABIN7354747)

Target: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADO antibody is conjugated to DyLight 550

Application: Flow Cytometry (FACS)

Product Details anti-ADO Antibody (DyLight 550)

Binding Specificity: AA 49-261

Purpose: Anti-Human ADO DyLight® 550 conjugated Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human ADO DyLight® 550 conjugated Antibody -Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human ADO recombinant protein (Position: E49-E261). Human ADO shares 90.1% amino acid (aa) sequence identity with mouse ADO.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Dominy, J. E., Jr., Simmons, C. R., Hirschberger, L. L., Hwang, J., Coloso, R. M., Stipanuk, M. H. Discovery and characterization of a second mammalian thiol dioxygenase, cysteamine dioxygenase.J. Biol. Chem. 282: 25189-25198, 2007.

Comment:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for ADO

Target: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Alternative Name: ADO

Background:

Synonyms: 2-aminoethanethiol dioxygenase, Cysteamine dioxygenase, ADO, C10orf22

Background: Human thiol dioxygenases include cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. It is demonstrated that mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. It is also noted that the demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.

Molecular Weight: 39 kDa