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TMEM166 antibody

FAM176A Reactivity: Human, Mouse, Rat WB, IHC (fro), IHC (p), FACS, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6719564
  • Target See all TMEM166 (FAM176A) Antibodies
    TMEM166 (FAM176A) (Family with Sequence Similarity 176, Member A (FAM176A))
    Reactivity
    • 31
    • 12
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 31
    • 2
    Rabbit
    Clonality
    • 33
    Polyclonal
    Conjugate
    • 12
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TMEM166 antibody is un-conjugated
    Application
    • 25
    • 13
    • 13
    • 6
    • 4
    • 3
    • 3
    • 1
    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC)
    Purpose
    Rabbit IgG polyclonal antibody for TMEM166/EVA1A detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human,Mouse,Rat.
    Sequence
    MRLPLSHSPE HVEMALLSNI LAAYSFVSEN PERA
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for TMEM166/EVA1A detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human,Mouse,Rat.
    Purification
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence of human TMEM166/EVA1A(MRLPLSHSPEHVEMALLSNILAAYSFVSENPERA).
    Isotype
    IgG
    Top Product
    Discover our top product FAM176A Primary Antibody
  • Application Notes

    Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Immunohistochemistry(Frozen Section)|0.5-1 μg/mL Immunocytochemistry|0.5-1 μg/mL Flow Cytometry|1-3 μg/1x106 cells

    Comment

    Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Buffer
    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Target
    TMEM166 (FAM176A) (Family with Sequence Similarity 176, Member A (FAM176A))
    Alternative Name
    EVA1A (FAM176A Products)
    Synonyms
    Fam176a antibody, RGD1559797 antibody, Tmem166 antibody, FAM176A antibody, TMEM166 antibody, BC014699 antibody, eva-1 homolog A, regulator of programmed cell death antibody, eva-1 homolog A (C. elegans) antibody, Eva1a antibody, EVA1A antibody
    Background

    Synonyms: Protein eva-1 homolog A, Protein FAM176A, Transmembrane protein 166, EVA1A, FAM176A, TMEM166, SP24

    Background: Eva-1 homolog A (C. elegans) is a protein that in humans is encoded by the EVA1A gene. It belongs to the FAM176 family. This gene is mapped to 2p12. EVA1A, also called TMEM166, acts as a regulator of programmed cell death, mediating both autophagy and apoptosis.

    Gene ID
    84141
    UniProt
    Q9H8M9
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