HMGN2 antibody

Catalog No. ABIN6719576

The Rabbit Polyclonal anti-HMGN2 antibody has been validated for IHC, WB, IF, ICC and FACS. It is suitable to detect HMGN2 in samples from Human.

Quick Overview for HMGN2 antibody (ABIN6719576)

Target: HMGN2 (High Mobility Group Nucleosomal Binding Domain 2 (HMGN2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGN2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-HMGN2 Antibody

Purpose: Anti-HMGN2 Antibody Picoband®

Sequence: KKGKADAGKE GNNPAENGDA KTDQAQKAEG AGDAK

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HMGN2 Antibody Picoband® (ABIN6719576). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HMGN2, which shares 94.3% and 88.6% amino acid (aa) sequence identity with mouse and rat HMGN2, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1. Porkka, K., Laakkonen, P., Hoffman, J. A., Bernasconi, M., Ruoslahti, E. A fragment of the HMGN2 protein homes to the nuclei of tumor cells and tumor endothelial cells in vivo. Proc. Nat. Acad. Sci. 99: 7444-7449, 2002. 2. West, K. L., Ito, Y., Birger, Y., Postnikov, Y., Shirakawa, H., Bustin, M.HMGN3a and HMGN3b, two protein isoforms with a tissue-specific expression pattern, expand the cellular repertoire of nucleosome-binding proteins. J. Biol. Chem. 276: 25959-25969, 2001.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HMGN2

Target: HMGN2 (High Mobility Group Nucleosomal Binding Domain 2 (HMGN2))

Alternative Name: HMGN2

Background:

Synonyms: NACHT, LRR and PYD domains-containing protein 2, Nucleotide-binding site protein 1, PYRIN domain and NACHT domain-containing protein 1, PYRIN-containing APAF1-like protein 2, NLRP2, NALP2, NBS1, PAN1, PYPAF2

Tissue Specificity: Expressed at high levels in lung, placenta and thymus and at lower levels in ovary, intestine and brain.

Background: Non-histone chromosomal protein HMG-17 is a protein that in humans is encoded by the HMGN2 gene. It is mapped to 1p36.11. The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMGN1, the encoded protein may help maintain an open chromatin configuration around transcribable genes. The protein has also been found to have antimicrobial activity against bacteria, viruses and fungi.

Molecular Weight: 18 kDa

Gene ID: 3151

UniProt: P05204