HOOK2 antibody (AA 500-702)

Catalog No. ABIN6719586

This anti-HOOK2 antibody is a Rabbit Polyclonal antibody detecting HOOK2 in WB, ELISA, IHC, ICC, IF and FACS. Suitable for Human.

Quick Overview for HOOK2 antibody (AA 500-702) (ABIN6719586)

Target: HOOK2 (Hook Homolog 2 (HOOK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HOOK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-HOOK2 Antibody

Binding Specificity: AA 500-702

Purpose: Anti-HOOK2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HOOK2 Antibody Picoband® (ABIN6719586). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HOOK2 recombinant protein (Position: E500-R702).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Kramer, H., Phistry, M. Genetic analysis of hook, a gene required for endocytic trafficking in Drosophila. Genetics 151: 675-684, 1999. 2. Walenta, J. H., Didier, A. J., Liu, X., Kramer, H. The Golgi-associated Hook3 protein is a member of a novel family of microtubule-binding proteins. J. Cell Biol. 152: 923-934, 2001.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HOOK2

Target: HOOK2 (Hook Homolog 2 (HOOK2))

Alternative Name: HOOK2

Background:

Synonyms: Protein Hook homolog 2, h-hook2, hHK2, HOOK2

Tissue Specificity: Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts.

Background: Protein Hook homolog 2 (HK2) is a protein that in humans is encoded by the HOOK2 gene. This gene is mapped to 19p13.13. Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.

Molecular Weight: 83 kDa

Gene ID: 29911