MR1 antibody (AA 23-269)

Catalog No. ABIN6719587

This Rabbit Polyclonal antibody specifically detects MR1 in ELISA, WB and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MR1 antibody (AA 23-269) (ABIN6719587)

Target: MR1 (Major Histocompatibility Complex, Class I-Related (MR1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MR1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-MR1 Antibody

Binding Specificity: AA 23-269

Purpose: Anti-MR1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MR1 Antibody (ABIN6719587). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MR1 recombinant protein (Position: R23-D269).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. "Entrez Gene: MR1 major histocompatibility complex, class I-related". 2. Hashimoto K, Hirai M, Kurosawa Y (Aug 1995). "A gene outside the human MHC related to classical HLA class I genes". Science. 269 (5224): 693-5. 3. Yamaguchi H, Kurosawa Y, Hashimoto K (Nov 1998). "Expanded genomic organization of conserved mammalian MHC class I-related genes, human MR1 and its murine ortholog". Biochem Biophys Res Commun. 250 (3): 558-64.

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MR1

Target: MR1 (Major Histocompatibility Complex, Class I-Related (MR1))

Alternative Name: MR1

Background:

Synonyms: Major histocompatibility complex class I-related gene protein, MHC class I-related gene protein, Class I histocompatibility antigen-like protein, MR1

Tissue Specificity: Ubiquitous.

Background: Major histocompatibility complex class I-related gene protein is a protein that in humans is encoded by the MR1 gene. MAIT (mucosal-associated invariant T-cells) lymphocytes represent a small population of T-cells primarily found in the gut. The protein encoded by this gene is an antigen-presenting molecule that presents metabolites of microbial vitamin B to MAITs. This presentation may activate the MAITs to regulate the amounts of specific types of bacteria in the gut. Several transcript variants encoding different isoforms have been found for this gene, and a pseudogene of it has been detected about 36 kbp upstream on the same chromosome.

Molecular Weight: 40 kDa

Gene ID: 3140

UniProt: Q95460

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints