SOX15 antibody (AA 1-233)

Catalog No. ABIN6719624

The Rabbit Polyclonal anti-SOX15 antibody has been validated for WB, ELISA, IHC, ICC, IF and FACS. It is suitable to detect SOX15 in samples from Human, Mouse and Rat.

Quick Overview for SOX15 antibody (AA 1-233) (ABIN6719624)

Target: SOX15 (SRY (Sex Determining Region Y)-Box 15 (SOX15))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-SOX15 Antibody

Binding Specificity: AA 1-233

Purpose: Anti-SOX15 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SOX15 Antibody Picoband® (ABIN6719624). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SOX15 recombinant protein (Position: M1-L233).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Cremazy, F., Soullier, S., Berta, P., Jay, P. Further complexity of the human SOX gene family revealed by the combined use of highly degenerate primers and nested PCR. FEBS Lett. 438: 311-314, 1998. 2. Lee, H.-J., Goring, W., Ochs, M., Muhlfeld, C., Steding, G., Paprotta, I., Engel, W., Adham, I. M.Sox15 is required for skeletal muscle regeneration. Molec. Cell. Biol. 24: 8428-8436, 2004. 

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SOX15

Target: SOX15 (SRY (Sex Determining Region Y)-Box 15 (SOX15))

Alternative Name: SOX15

Background:

Synonyms: Protein SOX-15, Protein SOX-12, Protein SOX-20, SOX15, SOX12, SOX20, SOX26, SOX27

Tissue Specificity: Widely expressed in fetal and adult tissues examined, highest level found in fetal spinal cord and adult brain and testis.

Background: Protein SOX-15 is a protein that in humans is encoded by the SOX15 gene. This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins.

Molecular Weight: 29 kDa

Gene ID: 6665

UniProt: O60248