SNRPN antibody (N-Term)

Catalog No. ABIN6719629

This Mouse Monoclonal antibody specifically detects SNRPN in WB and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SNRPN antibody (N-Term) (ABIN6719629)

Target: SNRPN (Small Nuclear Ribonucleoprotein Polypeptide N (SNRPN))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SNRPN antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 6F12

Product Details anti-SNRPN Antibody

Binding Specificity: AA 11-39, N-Term

Purpose: Anti-SNRPN Antibody Picoband® (monoclonal, 6F12)

Sequence: QHIDYRMRCI LQDGRIFIGT FKAFDKHMN

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SNRPN Antibody Picoband® (monoclonal, 6F12) (ABIN6719629). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SNRPN, identical to the related mouse and rat sequences.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1.Bielinska, B., Blaydes, S. M., Buiting, K., Yang, T., Krajewska-Walasek, M., Horsthemke, B., Brannan, C. I. De novo deletions of SNRPN exon 1 in early human and mouse embryos result in a paternal to maternal imprint switch. Nature Genet. 25: 74-78, 2000. 2.Cattanach, B. M., Barr, J. A., Evans, E. P., Burtenshaw, M., Beechey, C. V., Leff, S. E., Brannan, C. I., Copeland, N. G., Jenkins, N. A., Jones, J. A candidate mouse model for Prader-Willi syndrome which shows an absence of Snrpn expression. Nature Genet. 2: 270-274, 1992. 3.Geuns, E., De Rycke, M., Van Steirteghem, A., Liebaers, I. Methylation imprints of the imprint control region of the SNRPN-gene in human gametes and preimplantation embryos. Hum. Molec. Genet. 12: 2873-2879, 2003.

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SNRPN

Target: SNRPN (Small Nuclear Ribonucleoprotein Polypeptide N (SNRPN))

Alternative Name: SNRPN

Background:

Synonyms: Small nuclear ribonucleoprotein-associated protein N, snRNP-N, Sm protein D, Sm-D, Sm protein N, Sm-N, SmN, Tissue-specific-splicing protein, SNRPN, HCERN3, SMN

Tissue Specificity: Expressed in brain and lymphoblasts.

Background: SNRPN (Small Nuclear Ribonucleoprotein Polypeptide N), also called SMN, is a bicistronic imprinted gene that encodes 2 polypeptides, the SmN splicing factor, which is involved in RNA processing, and the SNRPN upstream reading frame (SNURF) polypeptide. The protein encoded by this gene is one polypeptide of a small nuclear ribonucleoprotein complex and belongs to the snRNP SMB/SMN family. SNRPN also encodes a long alternatively spliced transcript containing several small nucleolar RNAs (snoRNAs) and extends downstream to partially overlap the UBE3A gene in the antisense orientation. PWS arises from loss of function of genes in this region expressed exclusively from the paternal chromosome, suggesting that SNRPN may play a role in its etiology. The SNRPN gene is mapped on 15q11.2. Analysis of maternal DNA and of SNRPN cDNA confirmed that the maternal allele is not expressed in fetal brain and heart. Deletions in the transcription unit of the imprinted SNRPN gene occur in patients who have PWS or Angelman syndrome because of a parental imprint switch failure in this chromosomal domain.

Molecular Weight: 26 kDa

Gene ID: 6638

UniProt: P63162