TDRD3 antibody

Catalog No. ABIN6719636

The Rabbit Polyclonal anti-TDRD3 antibody has been validated for WB, IF and IHC. It is suitable to detect TDRD3 in samples from Human, Mouse and Rat.

Quick Overview for TDRD3 antibody (ABIN6719636)

Target: TDRD3 (Tudor Domain Containing 3 (TDRD3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TDRD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-TDRD3 Antibody

Purpose: Anti-TDRD3 Antibody Picoband®

Sequence: KPTNDNDEFE KQRTAAIAEV AKSKETK

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TDRD3 Antibody Picoband® (ABIN6719636). Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human TDRD3, which shares 96.3% and 100% amino acid (aa) sequence identity with mouse and rat TDRD3, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunofluorescence, 2 μg/mL
1. Goulet, Boisvenue, S., Mokas, S., Mazroui, R., Cote, J. TDRD3, a novel tudor domain-containing protein, localizes to cytoplasmic stress granules. Hum. Molec. Genet. 17: 3055-3074, 2008.  2. Linder, B., Plottner, O., Kroiss, M., Hartmann, E., Laggerbauer, B., Meister, G., Keidel, E., Fischer, U. Tdrd3 is a novel stress granule-associated protein interacting with the fragile-X syndrome protein FMRP. Hum. Molec. Genet. 17: 3236-3246, 2008.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TDRD3

Target: TDRD3 (Tudor Domain Containing 3 (TDRD3))

Alternative Name: TDRD3

Background:

Synonyms: Tudor domain-containing protein 3, TDRD3

Tissue Specificity: Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Background: Tudor domain-containing protein 3 is a protein that in humans is encoded by the TDRD3 gene. This gene is mapped to 13q21.2. It contains a Tudor domain and UBA protein domain and has three distinct Protein isoforms. TDRD3 is a multifunctional protein that acts as a transcriptional coactivator in the nucleus and as a scaffolding protein in cytoplasmic stress granules.

Molecular Weight: 65-73 kDa

Gene ID: 81550

UniProt: Q9H7E2