TMEM199 antibody (AA 20-129)

Catalog No. ABIN6719657

The Rabbit Polyclonal anti-TMEM199 antibody has been validated for WB and ELISA. It is suitable to detect TMEM199 in samples from Human, Mouse and Rat.

Quick Overview for TMEM199 antibody (AA 20-129) (ABIN6719657)

Target: TMEM199 (Transmembrane Protein 199 (TMEM199))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TMEM199 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TMEM199 Antibody

Binding Specificity: AA 20-129

Purpose: Anti-Transmembrane protein 199 TMEM199 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Transmembrane protein 199 TM Antibody (ABIN6719657). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TMEM199 recombinant protein (Position: E20-H129).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Calvo, P. L., Pagliardini, S., Baldi, M., Pucci, A., Sturiale, L., Garozzo, D., Vinciguerra, T., Barbera, C., Jaeken, J. Long-standing mild hypertransaminasaemia caused by congenital disorder of glycosylation (CDG) type IIx. J. Inherit. Metab. Dis. 31: S437-S440, 2008. 2. Jansen, J. C., Timal, S., van Scherpenzeel, M., Michelakakis, H., Vicogne, D., Ashikov, A., Moraitou, M., Hoischen, A., Huijben, K., Steenbergen, G., van den Boogert, M. A. W., Porta, F., and 14 others. TMEM199 deficiency is a disorder of Golgi homeostasis characterized by elevated aminotransferases, alkaline phosphatase, and cholesterol and abnormal glycosylation. Am. J. Hum. Genet. 98: 322-330, 2016.

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TMEM199

Target: TMEM199 (Transmembrane Protein 199 (TMEM199))

Alternative Name: TMEM199

Background:

Synonyms: Transmembrane protein 199, TMEM199, C17orf32

Tissue Specificity: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Background: TMEM199 encodes a protein homologous to the yeast V-ATPase assembly factor Vma12 and appears to be involved in Golgi homeostasis. The protein encoded by this gene has been observed to localize to the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC) and coat protein complex I (COPI) in some human cells. Defects in this gene are a cause of congenital disorder of glycosylation, type IIp. By genomic sequence analysis, the TMEM199 gene is mapped to chromosome 17q11.1.

Molecular Weight: 23 kDa

Gene ID: 147007

UniProt: Q8N511