Myc Tag antibody (AA 410-419) (DyLight 405)

Catalog No. ABIN6720638

Supplier Product No.: 600-446-381

The Rabbit Polyclonal anti-Myc Tag antibody is suitable to detect Myc Tag. It has been validated for WB, FLISA and FM.

Quick Overview for Myc Tag antibody (AA 410-419) (DyLight 405) (ABIN6720638)

Target: Myc Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myc Tag antibody is conjugated to DyLight 405

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Product Details anti-Myc Tag Antibody (DyLight 405)

Binding Specificity: AA 410-419

Supplier Product No.: 600-446-381

Supplier: Rockland

Purpose: MYC Epitope Tag Antibody Dylight™ 405 Conjugated

Cross-Reactivity (Details): The antibody recognizes the MYC epitope tag -tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Purification: This affinity purified antibody is directed against the MYC epitope tag and is useful in determining its presence in over expressed proteins in various assays.

Immunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with MYC epitope tag peptide, E-Q-K-L-I-S-E-E-D-L, conjugated to KLH using maleimide.  The sequence corresponds to amino acids 410-419 of human c-Myc.

Isotype: IgG

Labeling Ratio: 2.0

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000 - 1:25,000

Other: User Optimized

Comment:

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Myc Tag

Target: Myc Tag

Alternative Name: MYC Epitope Tag

Target Type: Tag

Background:

Synonyms: Rabbit anti-MYC Epitope Tag DyLight 405™ Conjugated Antibody, Rabbit anti-c-myc tag DyLight 405™ conjugation, Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu

Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Anti-MYC has utility to detect the fusion protein of the Myc epitope cloned along with the target gene. As such, anti-Myc/Myc can be used to identify fusion proteins containing the Myc epitope. This Anti-Myc antibody has been conjugated to DyLight™405.