DYKDDDDK Tag antibody (DyLight 649)

Catalog No. ABIN6720686

Supplier Product No.: 200-343-383

The Mouse Monoclonal anti-DYKDDDDK Tag antibody is suitable to detect DYKDDDDK Tag. It has been validated for WB, ELISA, FACS, FM, FLISA and MA.

Quick Overview for DYKDDDDK Tag antibody (DyLight 649) (ABIN6720686)

Target: DYKDDDDK Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This DYKDDDDK Tag antibody is conjugated to DyLight 649

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM), FLISA, Multiplex Assay (MA)

Clone: 29E4-G7

Product Details anti-DYKDDDDK Tag Antibody (DyLight 649)

Supplier Product No.: 200-343-383

Supplier: Rockland

Purpose: DYKDDDDK Tag (Anti-FLAG®) Antibody Dylight™ 649 Conjugated

Cross-Reactivity (Details): The antibody recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Purification: This antibody is directed against the FLAG™ epitope tag and is useful in determining its presence in over expressed proteins in various assays.

Immunogen: This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.

Isotype: IgG2a kappa

Labeling Ratio: 3.6

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

ELISA_Dilution: 1:10,000 - 1:25,000

Flow_Cytometry_Dilution: User Optimized

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000-1:25,000

Other: User Optimized

Comment:

Anti-FLAG DyLight™649 has been tested by ELISA, Western blot, and Flow Cytometry. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Background:

Synonyms: mouse anti-FLAG™ tag DyLight™649 conjugation, Enterokinase Cleavage Site (ECS), DyLight™649 conjugated mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys

Background: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.