DYKDDDDK Tag antibody (DyLight 800)

Catalog No. ABIN6720690

Supplier Product No.: 200-345-383

Cited in 1 publication. The DyLight 800-conjugated Mouse Monoclonal anti-DYKDDDDK Tag antibody (Clone 29E4-G7) (ABIN6720690) specifically detects DYKDDDDK Tag in WB, ELISA, FACS, DB, FM and FLISA.

Quick Overview for DYKDDDDK Tag antibody (DyLight 800) (ABIN6720690)

Target: DYKDDDDK Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This DYKDDDDK Tag antibody is conjugated to DyLight 800

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Dot Blot (DB), Fluorescence Microscopy (FM), FLISA

Clone: 29E4-G7

Product Details anti-DYKDDDDK Tag Antibody (DyLight 800)

Supplier Product No.: 200-345-383

Supplier: Rockland

Purpose: DYKDDDDK Tag (Anti-FLAG®) Antibody Dylight™ 800 Conjugated

Cross-Reactivity (Details): The antibody recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Purification: This antibody is directed against the FLAG™ epitope tag and is useful in determining its presence in over expressed proteins in various assays.

Immunogen: This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.

Isotype: IgG2a kappa

Labeling Ratio: 1.6

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

ELISA_Dilution: 1:10,000 - 1:25,000

Flow_Cytometry_Dilution: User Optimized

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000 - 1:25,000

Other: User Optimized

Comment:

Anti-FLAG DyLight™800 has been tested by ELISA, dot blot, and western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-DYKDDDDK Tag antibody (DyLight 800) (ABIN6720690)

Warmack, Pang, Peluso, Lowenson, Ong, Torres, Clarke: "Human Protein-l-isoaspartate O-Methyltransferase Domain-Containing Protein 1 (PCMTD1) Associates with Cullin-RING Ligase Proteins." in: Biochemistry, Vol. 61, Issue 10, pp. 879-894, (2022) (PubMed).

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Background:

Synonyms: mouse anti-FLAG™ tag DyLight™800 conjugation, Enterokinase Cleavage Site (ECS), DyLight™800 conjugated mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys

Background: Antibody for the detection of FLAG™ recognizes FLAG™ and is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. Antibody for the detection of FLAG™ can be used to identify fusion proteins containing the FLAG™ epitope. Antibody for the detection of FLAG™ recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.