NEFH antibody (AA 1-100)

Catalog No. ABIN672156

This anti-NEFH antibody is a Rabbit Polyclonal antibody detecting NEFH in WB, ELISA, IHC (p) and IF (p). Suitable for Human, Rat and Mouse. This Primary Antibody has been cited in 3+ publications.

Quick Overview for NEFH antibody (AA 1-100) (ABIN672156)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEFH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-NEFH Antibody

Binding Specificity: AA 1-100

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Neurofilament H

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-NEFH antibody (ABIN672156)

Zhao, Cheng, Du, Hou, Liu, Cui, Nie: "Transplantation of Cerebral Dopamine Neurotrophic Factor Transducted BMSCs in Contusion Spinal Cord Injury of Rats: Promotion of Nerve Regeneration by Alleviating Neuroinflammation." in: Molecular neurobiology, (2014) (PubMed).

Wang, Xu, Qin, Wang, Ma, Luo, Zhang: "Myocardin-related transcription factor-A is a key regulator in retinoic acid-induced neural-like differentiation of adult bone marrow-derived mesenchymal stem cells." in: Gene, Vol. 523, Issue 2, pp. 178-86, (2013) (PubMed).

Cheng, Liu, Zhao, Zhang, Guo, Nie: "Lentiviral-mediated transfer of CDNF promotes nerve regeneration and functional recovery after sciatic nerve injury in adult rats." in: Biochemical and biophysical research communications, Vol. 440, Issue 2, pp. 330-5, (2013) (PubMed).

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Alternative Name: Neurofilament H

Background:

Synonyms: NFH, Neurofilament heavy polypeptide, NF-H, 2 kDa neurofilament protein, Neurofilament triplet H protein, NEFH, KIAA845

Background: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

Gene ID: 4744

UniProt: P12036