Recombinant Histone H4 antibody (meLys20)

Catalog No. ABIN6731025

This Mouse Monoclonal antibody specifically detects Histone H4 in WB. It exhibits reactivity toward Human.

Quick Overview for Recombinant Histone H4 antibody (meLys20) (ABIN6731025)

Target: Histone H4 (HISTH4)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Histone H4 Antibody

Binding Specificity: meLys20

Brand: AbFlex®

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a peptide containing monomethyl-lysine 20 of human Histone H4.

Isotype: IgG2a

Application Details

Application Notes:
Validated Applications:
WB: 0.5 - 2 µg/ml

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet; therefore, we recommend a High Salt & Sonication Protocol when preparing nuclear extracts. In addition, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background. Individual optimization may be required.

Comment:

AbFlex^® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H4K20me1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in CHO cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for Histone H4

Target: Histone H4 (HISTH4)

Alternative Name: Histone H4

Molecular Weight: 12 kDa