Recombinant Nanog antibody (AA 2-25)

Catalog No. ABIN6731029

The Mouse Monoclonal anti-Nanog antibody has been validated for WB. It is suitable to detect Nanog in samples from Mouse.

Quick Overview for Recombinant Nanog antibody (AA 2-25) (ABIN6731029)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nanog Antibody

Binding Specificity: AA 2-25

Brand: AbFlex®

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a peptide comprising amino acids 2-25 of mouse Nanog.

Isotype: IgG2a

Application Details

Application Notes:
Validated Applications:
WB: 1 - 2 µg/mL

For optimal results, we recommend the addition of 0.1% Tween 20 to all Blotto solutions to reduce background. Individual optimization may be required.

Comment:

AbFlex^® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Nanog antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: Nanog

Molecular Weight: 45 kDa

Pathways: Stem Cell Maintenance