Recombinant KDM4B antibody

Catalog No. ABIN6731039

This anti-KDM4B antibody is a Mouse Monoclonal antibody detecting KDM4B in WB and IP. Suitable for Human.

Quick Overview for Recombinant KDM4B antibody (ABIN6731039)

Target: KDM4B (Lysine (K)-Specific Demethylase 4B (KDM4B))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KDM4B antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-KDM4B Antibody

Brand: AbFlex®

Purification: Protein A Chromatography

Immunogen: This antibody was raised against full-length recombinant human lysine-specific demethylase 4B (KDM4B) protein.

Isotype: IgG2a

Application Details

Application Notes:
Validated Applications:
IP: 4 µg per IP
WB*: 0.05 - 2 µg/ml

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet; therefore, we recommend a High Salt & Sonication Protocol when preparing nuclear extracts for Western Blot.

Comment:

AbFlex^® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex JMJD2B / KDM4B antibody was expressed in 293 cells and contains mouse immunoglobulin heavy and light chains.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for KDM4B

Target: KDM4B (Lysine (K)-Specific Demethylase 4B (KDM4B))

Alternative Name: JMJD2B / KDM4B

Molecular Weight: 130 kDa

Pathways: Warburg Effect