ADORA1 antibody (N-Term)

Catalog No. ABIN6735509

This anti-ADORA1 antibody is a Rabbit Polyclonal antibody detecting ADORA1 in WB and ELISA. Suitable for Rat.

Quick Overview for ADORA1 antibody (N-Term) (ABIN6735509)

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADORA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ADORA1 Antibody

Binding Specificity: N-Term

Specificity: Recognizes Adenosine A1 Receptor. Species cross-reactivity: Rat, porcine, lamb. Species sequence Homology: Mouse, canine, rabbit and human: 92 % , bovine: 78 %.

Purification: Protein A purified

Immunogen: Synthetic peptide 14aa sequence corresponding to rat A1R gene. This region corresponds to the 3rd extracellular domain, N-terminus. A cysteine has been added at the NH2 terminus for coupling to KLH.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), WB (1 - 10 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1-10 μg/mL using Detects 79 and 39kD bands in cortical membranes from rat, pig, and lamb. The 79kD band can be converted to 39kD band by agonist or antagonist treatment and could represent a A1R dimers. A1R, photoaffinity labeled with 125I-R-AHPIA as 39kD band. ELISA: 1:10000-1:50000 using 50-100 ng A1R control peptide/well.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 100 mM Tris,  pH 7.5, 0.2 % BSA, 0.05 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ADORA1

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Alternative Name: ADORA1 / Adenosine A1 Receptor

Background: Name/Gene ID: ADORA1
Subfamily: Adenosine
Family: GPCR

Synonyms: ADORA1, A1 adenosine receptor, A1ar, Adenosine A1 receptor, Adenosine receptor A1, ADO-A1 receptor, RDC7

Gene ID: 134

UniProt: P30542

Pathways: EGFR Signaling Pathway, Negative Regulation of Hormone Secretion, Synaptic Membrane