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ALT antibody (HRP)

The Sheep Polyclonal anti-ALT antibody has been validated for WB, IHC and ELISA. It is suitable to detect ALT in samples from Pig and Cow.
Catalog No. ABIN6735518

Quick Overview for ALT antibody (HRP) (ABIN6735518)

Target

See all ALT Antibodies
ALT (Alanine Aminotransferase (ALT))

Reactivity

  • 75
  • 35
  • 23
  • 18
  • 7
  • 6
  • 5
  • 4
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
Pig, Cow

Host

  • 93
  • 33
  • 4
  • 1
Sheep

Clonality

  • 95
  • 35
Polyclonal

Conjugate

  • 88
  • 22
  • 14
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This ALT antibody is conjugated to HRP

Application

  • 117
  • 81
  • 59
  • 47
  • 26
  • 14
  • 8
  • 8
  • 5
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
Western Blotting (WB), Immunohistochemistry (IHC), ELISA
  • Specificity

    Recognizes Alanine Transaminase. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-sheep serum as well as purified and partially purified Alanine transaminase (ALT) (Porcine Heart). Species cross-reactivity: Reported to cross-react with bovine ALT in Western Blot.

    Purification

    Ion exchange chromatography

    Immunogen

    Alanine Transaminase (ALT) from Porcine Heart.

    Type of Immunogen: Protein

    Isotype

    IgG
  • Application Notes

    Approved: ELISA (1:1000 - 1:3000), IHC, WB

    Usage: Suitable for use in Western Blot, Dot Blot, ELISA, Immunohistochemistry. ELISA: 1:1000-1:3000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS, pH 7.2, 10 mg/mL BSA, 0.01 % Gentamicin sulfate, 40 % glycerol.

    Preservative

    Gentamicin sulfate

    Precaution of Use

    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C
    Short term: +4°C. Avoid repeat freeze-thaw cycles.
  • Target

    ALT (Alanine Aminotransferase (ALT))

    Alternative Name

    GPT / Alanine Transaminase

    Background

    Name/Gene ID: GPT

    Synonyms: GPT, AAT1, ALT1, Alanine aminotransferase, Alanine aminotransferase 1, GPT 1, GPT1

    Gene ID

    2875

    UniProt

    P24298
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