Hephaestin antibody (C-Term)

Catalog No. ABIN6735670

The Rabbit Polyclonal anti-Hephaestin antibody has been validated for WB and ELISA. It is suitable to detect Hephaestin in samples from Mouse and Rat.

Quick Overview for Hephaestin antibody (C-Term) (ABIN6735670)

Target: Hephaestin (HEPH)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hephaestin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Hephaestin Antibody

Binding Specificity: C-Term

Specificity: The mouse sequence is 100 % conserved in rat and 94 % in human Hp. Control peptide, because of the small size (2-3kD), is not recommended for Western. It should be used in ELISA or antibody blocking (use 5-10 μg control peptide per 1 μg of IgG or 1 μL of antiserum) experiments to demonstrate antibody specificity.

Purification: Immunoaffinity purified

Immunogen: An 18-aa peptide sequence within the C-terminus of mouse/rat Hp (1) was synthesized, coupled to KLH.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB (1:1000 - 1:5000)

Usage: Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for antiserum and 1-10 μg/mL for aff. pure IgG using ECL. ELISA: ~1:100000 using 50-100 ng control peptide/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % BSA.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Hephaestin

Target: Hephaestin (HEPH)

Alternative Name: HEPH / Hephaestin

Background: Name/Gene ID: HEPH

Synonyms: HEPH, Hephaestin, CPL, KIAA0698

Gene ID: 9843

UniProt: Q9BQS7

Pathways: Transition Metal Ion Homeostasis