GABRA1 antibody (N-Term)

Catalog No. ABIN6735764

This anti-GABRA1 antibody is a Rabbit Polyclonal antibody detecting GABRA1 in WB, IHC and IHC (p). Suitable for Human, Cow, Rabbit and Monkey.

Quick Overview for GABRA1 antibody (N-Term) (ABIN6735764)

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Reactivity: Human, Cow, Rabbit, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GABRA1 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human GABRA1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rabbit (100%) Dog (92%) Mouse (91%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human GABRA1 (P14867, NP_000797). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Panda, Bovine, Rabbit (100%), Galago, Dog, Bat (92%), Mouse, Rat, Platypus (91%), Marmoset, Lizard (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GABRA1

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Alternative Name: GABRA1

Background: Name/Gene ID: GABRA1
Subfamily: GABA A receptor
Family: Ion Channel

Synonyms: GABRA1, GABA A receptor alpha 1, GABA(A) receptor, alpha 1, ECA4, EJM, EJM5

Gene ID: 2554

NCBI Accession: NP_000797

UniProt: P14867