MCOLN1 antibody (AA 101-150)

Catalog No. ABIN214172

The Rabbit Polyclonal anti-MCOLN1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect MCOLN1 in samples from Human, Dog, Pig, Rabbit, Bat and Monkey.

Quick Overview for MCOLN1 antibody (AA 101-150) (ABIN214172)

Target: MCOLN1 (Mucolipin 1 (MCOLN1))

Reactivity: Human, Dog, Pig, Rabbit, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCOLN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MCOLN1 Antibody

Binding Specificity: AA 101-150

Brand: IHC-plus™

Specificity: Human MCOLN1 / Mucolipin 1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Rabbit, Pig (100%) Rat, Bovine, Horse, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa101-150 of human MCOLN1 (Q9GZU1, NP_065394). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Dog, Bat, Rabbit, Pig (100%), Mouse, Rat, Bovine, Horse, Guinea pig (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (20 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MCOLN1

Target: MCOLN1 (Mucolipin 1 (MCOLN1))

Alternative Name: MCOLN1 / Mucolipin 1

Background: Name/Gene ID: MCOLN1
Subfamily: Calcium channel - TRP
Family: Ion Channel

Synonyms: MCOLN1, MLIV, ML4, Mucolipidin, Mucolipin 1, MSTP080, Mucolipidosis type IV protein, Mucolipin-1, TRP-ML1, TRPM-L1, MG-2, MST080, TRPML1

Gene ID: 57192

NCBI Accession: NP_065394

UniProt: Q9GZU1

Pathways: Transition Metal Ion Homeostasis