CXCL12 antibody (C-Term)

Catalog No. ABIN214748

The Rabbit Polyclonal anti-CXCL12 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect CXCL12 in samples from Human, Mouse, Dog, Chicken, Xenopus laevis, Bat, Guinea Pig, Horse and Monkey.

Quick Overview for CXCL12 antibody (C-Term) (ABIN214748)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reactivity: Human, Mouse, Dog, Chicken, Xenopus laevis, Bat, Guinea Pig, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CXCL12 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human CXCL12

Predicted Reactivity: Percent identity by BLAST analysis: Dog, Pig, Chicken (100%) Rat, Bovine (90%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human CXCL12 (P48061, NP_000600). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Dog, Cat, Bat, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus (100%), Rat, Bovine (90%), Elephant (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS buffer with 2 % sucrose

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CXCL12

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Alternative Name: SDF-1 / CXCL12

Background: Name/Gene ID: CXCL12
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: CXCL12, C-X-C motif chemokine 12, HIRH, IRH, SDF-1, SDF-1b, SDF1A, SDF1B, TPAR1, TLSF-a, TLSF-b, SDF-1a, SDF1, Sdf1alpha, Stromal cell-derived factor 1, TLSF, HSDF-1, PBSF, SCYB12

Gene ID: 6387

NCBI Accession: NP_000600

UniProt: P48061

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling