EIF4G2 antibody (AA 766-815)

Catalog No. ABIN6736375

This anti-EIF4G2 antibody is a Rabbit Polyclonal antibody detecting EIF4G2 in WB, IHC and IHC (p). Suitable for Human, Cow, Rabbit, Dog, Horse, Monkey, Pig and Bat.

Quick Overview for EIF4G2 antibody (AA 766-815) (ABIN6736375)

Target: EIF4G2 (Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2))

Reactivity: Human, Cow, Rabbit, Dog, Horse, Monkey, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4G2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EIF4G2 Antibody

Binding Specificity: AA 766-815

Brand: IHC-plus™

Specificity: Human EIF4G2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Horse, Pig (100%) Mouse, Rat, Guinea pig, Chicken (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa766-815 of human EIF4G2 (P78344). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig (100%), Mouse, Rat, Guinea pig, Turkey, Chicken (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EIF4G2

Target: EIF4G2 (Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2))

Alternative Name: DAP-5 / EIF4G2

Background: Name/Gene ID: EIF4G2

Synonyms: EIF4G2, AAG1, Aging-associated protein 1, DAP-5, DAP5, Death-associated protein 5, EIF-4G 2, EIF-4-gamma 2, EIF4G 2, Translation repressor nat1, p97

Gene ID: 1982