HIPK2 antibody (AA 384-433)

Catalog No. ABIN6736398

This Rabbit Polyclonal antibody specifically detects HIPK2 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Bat, Hamster, Monkey and Pig.

Quick Overview for HIPK2 antibody (AA 384-433) (ABIN6736398)

Target: HIPK2 (Homeodomain Interacting Protein Kinase 2 (HIPK2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Bat, Hamster, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIPK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HIPK2 Antibody

Binding Specificity: AA 384-433

Brand: IHC-plus™

Specificity: Human HIPK2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Guinea pig (100%) Rabbit, Chicken (92%) Zebrafish (86%) Bovine (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa384-433 of human HIPK2 (Q9H2X6, NP_073577). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Elephant, Opossum, Turkey, Chicken, Lizard (92%), Zebrafish (86%), Bovine (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.06 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HIPK2

Target: HIPK2 (Homeodomain Interacting Protein Kinase 2 (HIPK2))

Alternative Name: HIPK2

Background: Name/Gene ID: HIPK2
Subfamily: MNB/DYRK
Family: Protein Kinase

Synonyms: HIPK2, HHIPk2, PRO0593, Stank

Gene ID: 28996

NCBI Accession: NP_073577

UniProt: Q9H2X6

Pathways: Cell Division Cycle