Serotonin Receptor 3B antibody (N-Term)

Catalog No. ABIN6736401

The Rabbit Polyclonal anti-Serotonin Receptor 3B antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Serotonin Receptor 3B in samples from Human, Rat, Cow, Guinea Pig and Pig.

Quick Overview for Serotonin Receptor 3B antibody (N-Term) (ABIN6736401)

Target: Serotonin Receptor 3B (HTR3B)

Reactivity: Human, Rat, Cow, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 3B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Serotonin Receptor 3B Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human HTR3B / 5-HT3B

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human HTR3B (O95264, NP_006019). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Marmoset (100%), Orangutan, Monkey (92%), Elephant, Pig (85%), Panda (84%), Mouse, Rat (76%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Not recommended for: IF

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Serotonin Receptor 3B

Target: Serotonin Receptor 3B (HTR3B)

Alternative Name: HTR3B / 5-HT3B Receptor

Background: Name/Gene ID: HTR3B
Subfamily: Serotonin 5-HT3 receptor
Family: Ion Channel

Synonyms: HTR3B, 5-HT3b receptor, 5-HT3-B, 5-HT-3B, 5-HT3B, Serotonin receptor 3B

Gene ID: 9177

NCBI Accession: NP_006019

UniProt: O95264

Pathways: Synaptic Membrane