KCNK5 antibody (AA 421-470)

Catalog No. ABIN6736409

This Rabbit Polyclonal antibody specifically detects KCNK5 in WB, IHC, IF and IHC (p). It exhibits reactivity toward Human, Cow, Horse, Guinea Pig, Bat and Monkey.

Quick Overview for KCNK5 antibody (AA 421-470) (ABIN6736409)

Target: KCNK5 (Potassium Channel, Subfamily K, Member 5 (KCNK5))

Reactivity: Human, Cow, Horse, Guinea Pig, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNK5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KCNK5 Antibody

Binding Specificity: AA 421-470

Brand: IHC-plus™

Specificity: Human KCNK5

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine, Horse, Guinea pig (100%) Dog, Rabbit, Pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa421-470 of human KCNK5 (O95279, NP_003731). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Bat, Horse, Guinea pig (100%), Galago, Panda, Dog, Rabbit, Pig, Opossum (92%), Elephant (91%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IF (2.5 μg/mL), IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for KCNK5

Target: KCNK5 (Potassium Channel, Subfamily K, Member 5 (KCNK5))

Alternative Name: KCNK5 / TASK2

Background: Name/Gene ID: KCNK5
Subfamily: Potassium channel - two pore
Family: Ion Channel

Synonyms: KCNK5, K2p5.1, K2P5.1 potassium channel, TASK-2, TASK2

Gene ID: 8645

NCBI Accession: NP_003731

UniProt: O95279