SREBF2 antibody (AA 434-483)

Catalog No. ABIN6736431

This anti-SREBF2 antibody is a Rabbit Polyclonal antibody detecting SREBF2 in WB, IHC and IHC (p). Suitable for Human, Rat, Mouse, Pig, Dog, Cow, Guinea Pig, Horse, Chicken, Bat, Hamster, Monkey and Rabbit.

Quick Overview for SREBF2 antibody (AA 434-483) (ABIN6736431)

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Reactivity: Human, Rat, Mouse, Pig, Dog, Cow, Guinea Pig, Horse, Chicken, Bat, Hamster, Monkey, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SREBF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SREBF2 Antibody

Binding Specificity: AA 434-483

Brand: IHC-plus™

Specificity: Human SREBF2 / SREBP-2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Dog, Bovine, Horse, Guinea pig, Chicken (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa434-483 of human SREBF2 (Q12772, NP_004590). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Turkey, Chicken, Platypus (100%), Zebra finch, Lizard (93%), Xenopus (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/ul.Antibody has been tested in peptide-based ELISA.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SREBF2

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Alternative Name: SREBF2 / SREBP2

Background: Name/Gene ID: SREBF2

Synonyms: SREBF2, SREBP2, SREBP-2, BHLHd2

Gene ID: 6721

NCBI Accession: NP_004590

UniProt: Q12772

Pathways: Regulation of Lipid Metabolism by PPARalpha