TCF7 antibody (AA 228-277)

Catalog No. ABIN6736432

This Rabbit Polyclonal antibody specifically detects TCF7 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Cow, Guinea Pig, Horse, Rabbit, Monkey and Bat.

Quick Overview for TCF7 antibody (AA 228-277) (ABIN6736432)

Target: TCF7 (Transcription Factor 7 (T-Cell Specific, HMG-Box) (TCF7))

Reactivity: Human, Cow, Guinea Pig, Horse, Rabbit, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCF7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TCF7 Antibody

Binding Specificity: AA 228-277

Brand: IHC-plus™

Specificity: Human TCF7

Predicted Reactivity: Percent identity by BLAST analysis: Human, Horse, Guinea pig (100%) Mouse, Rat, Dog, Pig (92%) Bovine, Rabbit (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa228-277 of human TCF7 (Q86WR9, NP_003193). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Bat, Horse, Guinea pig (100%), Galago, Mouse, Rat, Panda, Dog, Pig (92%), Elephant, Bovine, Rabbit (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.12 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/ul.Antibody has been tested in peptide-based ELISA.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TCF7

Target: TCF7 (Transcription Factor 7 (T-Cell Specific, HMG-Box) (TCF7))

Alternative Name: TCF-1 / TCF7

Background: Name/Gene ID: TCF7
Family: Transcription factor

Synonyms: TCF7, TCF-7, TCF1, Transcription factor 7, T-cell factor 1, TCF-1

Gene ID: 6932

NCBI Accession: NP_003193

UniProt: P36402

Pathways: WNT Signaling