TRIM13 antibody (AA 165-214)

Catalog No. ABIN6736435

The Rabbit Polyclonal anti-TRIM13 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect TRIM13 in samples from Human, Rat, Cow, Guinea Pig, Horse, Rabbit, Bat, Hamster, Monkey and Pig.

Quick Overview for TRIM13 antibody (AA 165-214) (ABIN6736435)

Target: TRIM13 (Tripartite Motif Containing 13 (TRIM13))

Reactivity: Human, Rat, Cow, Guinea Pig, Horse, Rabbit, Bat, Hamster, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM13 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TRIM13 Antibody

Binding Specificity: AA 165-214

Brand: IHC-plus™

Specificity: Human TRIM13 / RFP2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Orangutan, Rat, Bovine (100%) Mouse (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa165-214 of human TRIM13 (Q5UBW0, NP_001007279). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Rat, Hamster, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus (100%), Mouse, Dog (92%), Turkey, Zebra finch, Chicken (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.12 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Antibody was tested in a peptide-based ELISA.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TRIM13

Target: TRIM13 (Tripartite Motif Containing 13 (TRIM13))

Alternative Name: TRIM13

Background: Name/Gene ID: TRIM13
Family: Tripartite Motif

Synonyms: TRIM13, DLEU5, CLL-associated RING finger, LEU5, Leukemia-associated protein 5, RING finger protein 77, Tripartite motif protein 13, Putative tumor suppressor RFP2, RFP2, CAR, Ret finger protein 2, RNF77, Tripartite motif containing 13, Tripartite motif-containing 13

Gene ID: 10206

NCBI Accession: NP_001007279

UniProt: O60858