TRIM17 / RNF16 antibody (AA 254-303)

Catalog No. ABIN6736437

The Rabbit Polyclonal anti-TRIM17 / RNF16 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect TRIM17 / RNF16 in samples from Human.

Quick Overview for TRIM17 / RNF16 antibody (AA 254-303) (ABIN6736437)

Target: TRIM17 / RNF16 (TRIM17)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM17 / RNF16 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TRIM17 / RNF16 Antibody

Binding Specificity: AA 254-303

Brand: IHC-plus™

Specificity: Human TRIM17

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa254-303 of human TRIM17 (Q9Y577, NP_001020111). Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%), Marmoset (92%), Gibbon, Rabbit (85%), Rat (78%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.12 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TRIM17 / RNF16

Target: TRIM17 / RNF16 (TRIM17)

Alternative Name: TRIM17 / RNF16

Background: Name/Gene ID: TRIM17
Family: Tripartite Motif

Synonyms: TRIM17, Tripartite motif containing 17, Tripartite motif-containing 17, RNF16, RBCC, RING finger protein 16, RING finger protein terf, Terf, Testis RING finger protein

Gene ID: 51127

NCBI Accession: NP_001020111

UniProt: Q9Y577