TRIM34 antibody (AA 49-98)

Catalog No. ABIN6736440

The Rabbit Polyclonal anti-TRIM34 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect TRIM34 in samples from Human.

Quick Overview for TRIM34 antibody (AA 49-98) (ABIN6736440)

Target: TRIM34 (Tripartite Motif Containing 34 (TRIM34))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM34 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TRIM34 Antibody

Binding Specificity: AA 49-98

Brand: IHC-plus™

Specificity: Human TRIM34 / RNF21

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa49-98 of human TRIM34 (Q9BYJ4-2, NP_569074). Percent identity by BLAST analysis: Human (100%), Baboon, Monkey (92%), Gibbon (91%), Marmoset (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TRIM34

Target: TRIM34 (Tripartite Motif Containing 34 (TRIM34))

Alternative Name: TRIM34 / RNF21

Background: Name/Gene ID: TRIM34
Family: Tripartite Motif

Synonyms: TRIM34, IFP1, RING finger protein 21, RNF21, Tripartite motif containing 34, Tripartite motif-containing 34

Gene ID: 53840

NCBI Accession: NP_569074

UniProt: Q9BYJ4