TRIM9 antibody (C-Term)

Catalog No. ABIN6736443

The Rabbit Polyclonal anti-TRIM9 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect TRIM9 in samples from Human.

Quick Overview for TRIM9 antibody (C-Term) (ABIN6736443)

Target: TRIM9 (Tripartite Motif Containing 9 (TRIM9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TRIM9 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human TRIM9

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Rabbit (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human TRIM9 (Q9C026-5, NP_443210). Percent identity by BLAST analysis: Human, Chimpanzee (100%), Rabbit (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TRIM9

Target: TRIM9 (Tripartite Motif Containing 9 (TRIM9))

Alternative Name: SPRING / TRIM9

Background: Name/Gene ID: TRIM9
Family: Tripartite Motif

Synonyms: TRIM9, KIAA0282, RING finger protein 91, SPRING, Tripartite motif containing 9, RNF91, Tripartite motif-containing 9

Gene ID: 114088

NCBI Accession: NP_443210

UniProt: Q9C026

Pathways: Synaptic Vesicle Exocytosis