USP3 antibody (AA 50-99)

Catalog No. ABIN6736444

This Rabbit Polyclonal antibody specifically detects USP3 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Bat and Monkey.

Quick Overview for USP3 antibody (AA 50-99) (ABIN6736444)

Target: USP3 (Ubiquitin Specific Peptidase 3 (USP3))

Reactivity: Human, Mouse, Rat, Dog, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-USP3 Antibody

Binding Specificity: AA 50-99

Brand: IHC-plus™

Specificity: Human USP3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog (100%) Bovine, Horse (92%) Xenopus (90%) Mouse, Rat, Chicken (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa50-99 of human USP3 (Q9Y6I4, NP_006528). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Rat, Elephant, Panda, Dog, Bat (100%), Galago, Bovine, Rabbit, Horse, Guinea pig (92%), Xenopus (90%), Mouse, Opossum, Turkey, Zebra finch, Chicken, Platypus (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for USP3

Target: USP3 (Ubiquitin Specific Peptidase 3 (USP3))

Alternative Name: USP3 / UBP

Background: Name/Gene ID: USP3
Subfamily: Cysteine C19
Family: Protease

Synonyms: USP3, Deubiquitinating enzyme 3, SIH003, Ubiquitin specific protease 3, UBP, Ubiquitin thiolesterase 3, Ubiquitin thioesterase 3, Ubiquitin specific peptidase 3

Gene ID: 9960

NCBI Accession: NP_006528

UniProt: Q9Y6I4