ZBTB33 antibody (N-Term)

Catalog No. ABIN6736447

This Rabbit Polyclonal antibody specifically detects ZBTB33 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Dog, Horse, Cow, Hamster, Guinea Pig, Monkey and Pig.

Quick Overview for ZBTB33 antibody (N-Term) (ABIN6736447)

Target: ZBTB33 (Zinc Finger and BTB Domain Containing 33 (ZBTB33))

Reactivity: Human, Dog, Horse, Cow, Hamster, Guinea Pig, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZBTB33 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ZBTB33 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human ZBTB33 / Kaiso

Predicted Reactivity: Percent identity by BLAST analysis: Dog, Bovine, Horse, Pig (100%) Mouse, Rat, Rabbit (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human ZBTB33 (Q86T24, NP_006768). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Hamster, Panda, Dog, Bovine, Horse, Pig, Guinea pig (100%), Mouse, Rat, Rabbit, Opossum (92%), Chicken, Platypus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ZBTB33

Target: ZBTB33 (Zinc Finger and BTB Domain Containing 33 (ZBTB33))

Alternative Name: ZBTB33 / Kaiso

Background: Name/Gene ID: ZBTB33

Synonyms: ZBTB33, Kaiso, WUGSC:H_DJ525N14.1, ZNF-kaiso, Kaiso transcription factor, ZNF348

Gene ID: 10009

NCBI Accession: NP_006768

UniProt: Q86T24