Lipin 1 antibody (AA 111-126)
Quick Overview for Lipin 1 antibody (AA 111-126) (ABIN6736492)
Target
See all Lipin 1 (LPIN1) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 111-126
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Specificity
- Recognizes mouse Lipin 1. Species sequence Homology: rat (93 % ), human and chimpanzee (81 %), chicken (75 %). Epitope is around 120aa region that is well before the truncation site for isoform 1, therefore epitope is present in both isoforms 1 and 2. No significant sequence homology seen with Lipin-2, Lipin-3 or other proteins.
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Purification
- Immunoaffinity purified
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Immunogen
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Synthetic 16aa peptide sequence (aa111-126) at the N-terminus of mouse Lipin 1 (KLH coupled).
Type of Immunogen: Synthetic peptide -
Isotype
- IgG
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Application Notes
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Approved: ELISA (0.5 - 1 μg/mL), IF, WB (1 - 10 μg/mL)
Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1-10 μg/mL using ECL. Will recognize ~140kD mouse Lipin-1 under non-reducing conditions. It is not clear if isoform 2 is also recognized with this antibody. ELISA: 0.5-1 μg/mL. Control peptide can be used to coat ELISA plates at 1 μg/mL. -
Comment
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Target Species of Antibody: Mouse
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- Supplied as liquid in PBS, pH 7.5, 0.1 % BSA, 40 % glycerol.
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Handling Advice
- Avoid repeat freeze-thaw cycles.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Lipin 1 (LPIN1)
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Alternative Name
- LPIN1 / Lipin 1
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Background
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Name/Gene ID: LPIN1
Synonyms: LPIN1, Lipin-1, Lipin 1, PAP1, KIAA0188 -
Gene ID
- 23175
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UniProt
- Q14693
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Pathways
- Monocarboxylic Acid Catabolic Process
Target
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