Sonic Hedgehog antibody (N-Term)

Catalog No. ABIN6736667

The Rabbit Polyclonal anti-Sonic Hedgehog antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Sonic Hedgehog in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Pig, Dog, Chicken, Guinea Pig, Horse, Xenopus laevis, Hamster and Bat.

Quick Overview for Sonic Hedgehog antibody (N-Term) (ABIN6736667)

Target: Sonic Hedgehog (SHH)

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Pig, Dog, Chicken, Guinea Pig, Horse, Xenopus laevis, Hamster, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sonic Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Sonic Hedgehog Antibody

Binding Specificity: N-Term

Specificity: Human SHH

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%) Bovine (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human SHH (Q15465, NP_000184). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Dog, Bat, Horse, Pig, Opossum, Guinea pig, Chicken, Lizard, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish (100%), Marmoset, Bovine (92%), Zebra finch (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB (0.2 - 1 μg/mL)

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Alternative Name: Sonic Hedgehog / SHH

Background: Name/Gene ID: SHH
Subfamily: Cysteine C46
Family: Protease

Synonyms: SHH, HHG-1, HLP3, Sonic hedgehog, Sonic hedgehog homolog, MCOPCB5, Sonic hedgehog protein, HHG1, HPE3, SMMCI, TPT, TPTPS

Gene ID: 6469

NCBI Accession: NP_000184

UniProt: Q15465

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development