HFE antibody (AA 149-198)

Catalog No. ABIN6736669

The Rabbit Polyclonal anti-HFE antibody is suitable to detect HFE in samples from Human, Rat, Dog and Monkey. It has been validated for WB, IHC and IHC (p).

Quick Overview for HFE antibody (AA 149-198) (ABIN6736669)

Target: HFE (Hemochromatosis (HFE))

Reactivity: Human, Rat, Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HFE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HFE Antibody

Binding Specificity: AA 149-198

Specificity: Human HFE

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog (100%) Bovine, Pig (92%) Guinea pig (91%) Rat (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa149-198 of human HFE (Q30201-4, NP_620577). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Dog (100%), Galago, Bovine, Pig (92%), Guinea pig (91%), Elephant, Bat, Horse (85%), Rat (84%), Orangutan, Opossum (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB (0.2 - 1 μg/mL)

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HFE

Target: HFE (Hemochromatosis (HFE))

Alternative Name: HFE

Background: Name/Gene ID: HFE

Synonyms: HFE, HFE1, HH, MHC class I-like protein HFE, Hemochromatosis, High Fe, MVCD7, HLA-H, HLAH, TFQTL2

Gene ID: 3077

NCBI Accession: NP_620577

UniProt: Q30201

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process