AKAP7 antibody (N-Term)

Catalog No. ABIN6736700

This Rabbit Polyclonal antibody specifically detects AKAP7 in WB, IHC and IHC (p). It exhibits reactivity toward Human and Monkey.

Quick Overview for AKAP7 antibody (N-Term) (ABIN6736700)

Target: AKAP7 (A Kinase (PRKA) Anchor Protein 7 (AKAP7))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKAP7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-AKAP7 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human AKAP7

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human AKAP7 (O43687, NP_619539). Percent identity by BLAST analysis: Human, Gibbon, Marmoset (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for AKAP7

Target: AKAP7 (A Kinase (PRKA) Anchor Protein 7 (AKAP7))

Alternative Name: AKAP7

Background: Name/Gene ID: AKAP7

Synonyms: AKAP7, A-kinase anchor protein 9 kDa, A-kinase anchor protein 18 kDa, AKAP-7 isoforms alpha and beta, AKAP-7 isoform gamma, AKAP 18, AKAP15, AKAP18, PRKA7 isoform gamma, PRKA7 isoforms alpha/beta

Gene ID: 9465

NCBI Accession: NP_619539

UniProt: Q9P0M2