DDX39B antibody (C-Term)

Catalog No. ABIN6736703

This Rabbit Polyclonal antibody specifically detects DDX39B in IHC, WB and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Goat, Guinea Pig, Horse, Monkey, Pig, Rabbit, Bat, Chicken and Dog.

Quick Overview for DDX39B antibody (C-Term) (ABIN6736703)

Target: DDX39B (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 39B (DDX39B))

Reactivity: Human, Mouse, Rat, Cow, Goat, Guinea Pig, Horse, Monkey, Pig, Rabbit, Bat, Chicken, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX39B antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DDX39B Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human DDX39B / UAP56

Predicted Reactivity: Percent identity by BLAST analysis: Human, Goat (100%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human DDX39B (Q13838, NP_542165). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Goat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Turkey, Chicken, Platypus (100%), Opossum, Lizard, Xenopus, Zebrafish, Drosophila (92%), Salmon, Smelt, Nematode (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for DDX39B

Target: DDX39B (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 39B (DDX39B))

Alternative Name: DDX39B / UAP56 / BAT1

Background: Name/Gene ID: DDX39B

Synonyms: DDX39B, BAT1, DEAD box protein UAP56, HLA-B associated transcript 1, UAP56, ATP-dependent RNA helicase p47, D6S81E, Spliceosome RNA helicase BAT1

Gene ID: 7919

NCBI Accession: NP_542165

UniProt: Q13838

Pathways: Ribonucleoprotein Complex Subunit Organization