Chromogranin A antibody (C-Term)

Catalog No. ABIN214090

This Rabbit Polyclonal antibody specifically detects Chromogranin A in IHC, WB and IHC (p). It exhibits reactivity toward Human, Rat, Mouse, Monkey, Pig, Cow, Horse, Rabbit, Dog, Guinea Pig and Hamster.

Quick Overview for Chromogranin A antibody (C-Term) (ABIN214090)

Target: Chromogranin A (CHGA)

Reactivity: Human, Rat, Mouse, Monkey, Pig, Cow, Horse, Rabbit, Dog, Guinea Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Chromogranin A antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Chromogranin A Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human CHGA / Chromogranin A

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Horse, Pig, Guinea pig (100%) Chicken (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human CHGA (P10645, NP_001266). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Turkey, Zebra finch, Chicken, Lizard (92%), Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Chromogranin A

Target: Chromogranin A (CHGA)

Alternative Name: CHGA / Chromogranin A

Background: Name/Gene ID: CHGA

Synonyms: CHGA, Chromogranin A, Chromogranin-A, Pancreastatin, Pituitary secretory protein I, Vasostatin I, SP-I, Vasostatin II

Gene ID: 1113

NCBI Accession: NP_001266

UniProt: P10645

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling