CPA1 antibody (AA 24-73)

Catalog No. ABIN6736707

The Rabbit Polyclonal anti-CPA1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect CPA1 in samples from Human, Cow, Dog and Horse.

Quick Overview for CPA1 antibody (AA 24-73) (ABIN6736707)

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Reactivity: Human, Cow, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CPA1 Antibody

Binding Specificity: AA 24-73

Brand: IHC-plus™

Specificity: Human CPA1

Predicted Reactivity: Percent identity by BLAST analysis: Pig (92%) Dog (85%) Bovine (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa24-73 of human CPA1 (P15085, NP_001859). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Horse (100%), Monkey, Rat, Bat, Pig (92%), Marmoset, Dog (85%), Bovine (83%), Mouse (78%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CPA1

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Alternative Name: CPA1 / Carboxypeptidase A

Background: Name/Gene ID: CPA1
Subfamily: Metallopeptidase M14
Family: Exopeptidase

Synonyms: CPA1, CPA, Carboxypeptidase A1, Pancreatic carboxypeptidase A, Carboxypeptidase A

Gene ID: 1357

NCBI Accession: NP_001859

UniProt: P15085