DIDO1 antibody (AA 72-121)

Catalog No. ABIN6736710

The Rabbit Polyclonal anti-DIDO1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect DIDO1 in samples from Human, Guinea Pig, Rabbit and Monkey.

Quick Overview for DIDO1 antibody (AA 72-121) (ABIN6736710)

Target: DIDO1 (Death Inducer-Obliterator 1 (DIDO1))

Reactivity: Human, Guinea Pig, Rabbit, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIDO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DIDO1 Antibody

Binding Specificity: AA 72-121

Brand: IHC-plus™

Specificity: Human DIDO1 / DATF1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rabbit (100%) Mouse, Rat, Bovine, Horse, Chicken (92%) Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa72-121 of human DIDO1 (Q9BTC0-2, NP_542987). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Rabbit (100%), Mouse, Rat, Bovine, Horse, Chicken (92%), Opossum, Guinea pig (85%), Galago, Elephant, Bat, Turkey, Lizard (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for DIDO1

Target: DIDO1 (Death Inducer-Obliterator 1 (DIDO1))

Alternative Name: DATF1 / DIDO1

Background: Name/Gene ID: DIDO1
Family: Transcription factor

Synonyms: DIDO1, C20orf158, Death-inducer obliterator 1, DIDO3, DIDO2, DJ885L7.8, DIO-1, HDido1, KIAA0333, BYE1, DATF-1, DATF1, Death inducer-obliterator 1

Gene ID: 11083

NCBI Accession: NP_542987

UniProt: Q9BTC0

Pathways: Hormone Activity