EGR4 antibody (C-Term)

Catalog No. ABIN214101

This anti-EGR4 antibody is a Rabbit Polyclonal antibody detecting EGR4 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Chicken, Monkey and Pig.

Quick Overview for EGR4 antibody (C-Term) (ABIN214101)

Target: EGR4 (Early Growth Response 4 (EGR4))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Chicken, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGR4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EGR4 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human EGR4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human EGR4 (Q05215, NP_001956). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Turkey, Chicken (100%), Opossum (92%), Medaka, Drosophila, Water flea (90%), Xenopus (84%), Tamarin, Panda, Bat, Platypus (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.06 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EGR4

Target: EGR4 (Early Growth Response 4 (EGR4))

Alternative Name: EGR4 / EGR-4

Background: Name/Gene ID: EGR4

Synonyms: EGR4, AT133, Early growth response 4, NGFIC, NGFI-C, EGR-4, PAT133

Gene ID: 1961

NCBI Accession: NP_001956

UniProt: Q05215