ETV4 antibody (AA 181-230)

Catalog No. ABIN214103

The Rabbit Polyclonal anti-ETV4 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect ETV4 in samples from Human, Mouse, Rat, Cow and Pig.

Quick Overview for ETV4 antibody (AA 181-230) (ABIN214103)

Target: ETV4 (Ets Variant 4 (ETV4))

Reactivity: Human, Mouse, Rat, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ETV4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ETV4 Antibody

Binding Specificity: AA 181-230

Brand: IHC-plus™

Specificity: Human ETV4 / PEA3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Pig (100%) Rabbit, Horse (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa181-230 of human ETV4 (P43268, NP_001977). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Mouse, Rat, Elephant, Bovine, Pig (100%), Marmoset, Bat, Rabbit, Horse (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ETV4

Target: ETV4 (Ets Variant 4 (ETV4))

Alternative Name: PEA3 / ETV4

Background: Name/Gene ID: ETV4

Synonyms: ETV4, ETS translocation variant 4, E1A-F, E1AF, Ets variant 4, PEA3, Protein PEA3, PEAS3

Gene ID: 2118

NCBI Accession: NP_001977

UniProt: P43268